Nairo Quintana charged to top of the Giro d'Italia general classification on Sunday after an accident with a stray police motorbike ended the hopes of Geraint Thomas and Adam Yates.
A police motorbike stopped at the side of the road ahead of the charging peloton at the base of the crucial Blockhaus climb caused a crash.
Both Yates and Thomas were able to resume the race, but they face an uphill struggle to reel Quintana in now, while Team Sky's other GC hope Mikel Landa is also adrift following the crash.
Quintana stamped his authority on the Giro when he claimed the overall lead by winning the first big mountain stage, while defending champion Vincenzo Nibali was dealt a big knock when he cracked on the climb.
Yates finished nearly five minutes behind Quintana on the day, while Thomas was slightly further back as both saw their hopes end.
Bauke Mollema (Trek-Segafredo) is 51 seconds behind in fourth and Nibali is fifth, 1min 10secs behind.
Former race leader Bob Jungels also suffered on the final climb as the Quick-Step Floors rider gave up the pink jersey after finishing over three minutes behind Quintana.
"I don't know what to say, except it's ridiculous", said Thomas, whose shoulder "popped out" in the spill before the final climb to the summit finish of Blockhaus on stage nine.
"I'm a bit angry at the minute".
"The bike had just stopped on the side of the road, we were all racing for position and someone in front of me hit it and we had nowhere to go".
"I felt good, then I crashed".
Quintana, the Colombian who won the 2014 Giro and was twice runner-up in the Tour de France, finished 24 seconds ahead of Thibaut Pinot of France, who edged Tom Dumoulin of the Netherlands in a sprint for second. I'm sure the guy who was riding the motorbike realises that too.
"But I do think we need to go back and have a look at it and ask the question, 'Why did it happen?' - but we fight on".
Team Orica-Scott rider Yates, from Yorkshire, is now in 16th place, four minutes and 49 seconds off the leader.
There is a rest day tomorrow, before Tuesday's stage 10, a 39.8km individual time trial from Foligno to Montefalco.